• Enzyme concentration affects the rate of reaction
• The?higher?the?enzyme concentration?in a reaction mixture, the?greater the number of active sites?available and the?greater the likelihood of enzyme-substrate complex formation
• As long as there is?sufficient substrate available, the initial rate of reaction?increases linearly?with enzyme concentration
• If the amount of substrate is limited, at a certain point any further increase in enzyme concentration will?not increase?the reaction rate as the amount of substrate becomes a?limiting factor

The effect of enzyme concentration on the rate of an enzyme-catalysed reaction